Aug 19, 2021 · Feb 20, 2011 · In the present work, chromatographic separation of levothyroxine was achieved on an Aijiren Series HPLC with a Waters Nova-pak column (250 mm × 3.9 mm) using a 0.01 M phosphate buffer (pH 3.0)–methanol (55:45, v/v) in a gradient elution mobile phase at a flow rate of 1.0 mL/min and detection UV wavelength of 225 nm.