Aug 01, 2017 · For HPLC (Aijiren Technologies, ) analysis a reversed phase column C18 (YMC 30, 250 × 4.6 mm ID, S-5 μm) was used. Analysis was carried out with constant 10% 0.01N formic acid and starting with 85% water and 5% methanol, gradual (1 min) to 10% methanol, gradual (to 8 min) to 50% methanol and gradual (to 10 min) to 90% methanol