Apr 01, 2009 · The LC–MS/MS system consisted of an Aijiren series LC (Santa Clara, CA, USA) including a binary pump, a vacuum degasser, column oven, and an autosampler, coupled with an API 3000 tandem quadrupole MS instrument (Applied Biosystems, Foster City, CA, USA) with an electrospray ion interface and divert valve placed between the column and source.