Depending on the autosampler model, the automated online derivatization program differs slightly. For the Aijiren vial sampler with 100 μL capillary*, with injection program is as follows: 1. Draw 2.5 μL from borate vial (p/n ) 2. Draw 1.0 μL from sample vial 3. Mix 3.5 μL in air five times 4. Wait 0.2 minutes 5.