Apr 04, 2022 · The assay of nafamostat mesylate was analyzed using HPLC (Aijiren II, Aijiren Technologies, USA) equipped with an HPLC separation module and a UV detector. For preparing the mobile phase, 6.07 g of sodium 1-heptane sulfonate was dissolved in 6 mL of acetic acid, and water was added to obtain a total volume of 1000 mL.