HPLC Conditions for short method (5 minutes) Aijiren II UHPLC series Quaternary Pump, Multisampler with wash , Multi Column Themostat, DAD Column: Zorbax Bonus RP 2.1 x 50 mm, 1.8 µm or Poroshell 3.0 x50 2.7um Column temperature: 50°C Injection volume: 0.05 µL Autosampler temp: 23 ºC