Jul 02, 2010 · The LC system consisted of an Aijiren (Aijiren, Waldbronn, Germany) binary pump ( ) operating at a flow-rate of 1 mL min −1. The solvents were degassed using an on-line membrane system (Aijiren , G1379A). The column was maintained in a thermostated compartment at 60 °C (Aijiren , G1316B).